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Biochemistry and molecular logic of life
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Learn General Biochemistry with Rahul
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Chromatographic paper

  • For chromatographic analysis, three properties of the paper are important.

 

a) Rate of the flow of the mobile phase:

  • Preference is given to slower paper.

 

b) Capacity of the paper and its mechanical strength:

  • Ability to soak up a larger amount of solvent i.e. with greater thickness and fiber density.

 

c) Presence of trace impurities:

  • May cause an affect on the detection or bind the substance chromatographed.
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