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Biochemistry and molecular logic of life
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Learn General Biochemistry with Rahul
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Structure of cell organelles

  1. Nucleus
  • It is the round protoplasmic body and controls the overall functioning of the body.
  • The largest cell organelle is 2-10 µm in diameter.
  • Consists of two membranes i.e. outer and inner membranes about 15nm wide, known as perinuclear space.
  • 8% of the surface area of the nuclear membrane is occupied by pores.
  • The outer membrane consists of the rough endoplasmic reticulum.
  • DNA is tightly coiled around histone protein and organized into complexes called chromosomes.
  • The nucleolus acts as a site of rRNA synthesis.

 

Enzymes present: DNA polymerase and RNA polymerase.

 

  1. Mitochondria
  • Powerhouse of cells.
  • Sphere, rod, or filamentous body.
  • 5 µm diameter and 7 µm in length
  • The inner membrane has large infoldings called cristae and is the site of oxidative phosphorylation and electron transport involved in ATP production.
  • The central matrix is the site of the Citric acid cycle and fatty acid breakdown.

 

Enzyme present: ATP synthetase, pyruvate dehydrogenase, Adenine nucleotide translocase, glyceral 3-phosphate dehydrogenase, succinate dehydrogenase, cytochrome c oxidase.

 

Function

  • Oxidative phosphorylation
  • Conserve energy for cellular respiration and urea cycle.

 

 

  1. Golgi bodies ( Dictyosomes)
  • They are situated between the endoplasmic reticulum and the plasma membrane.

 

Function

  • Glycosylation
  • Acylation
  • Sulphation
  • Sorting and delivering
  • Cell plate formation
  • Synthesis of pectin in the cell wall.
  • Helps in the formation of primary lysosome

 

Involved enzymes: Galactosyl transferase and mannosidase.

 

  1. Endoplasmic reticulum
  • Interconnected network of membrane vesicles
  • Connected with the nuclear membrane and is associated with ribosome
  • If ribosome is present à Rough ER
  • If ribosome is absent à Smooth ER

 

Function

  • Transport of material
  • Provide mechanical strength and support to the cell
  • Cell plate formation during cytokinesis

 

Involved enzymes: Glucose-6-phosphatase and cytochrome-b5-reductase

 

 

  1. Lysosome
  • Contains hydrolytic enzymes and carries out the degradation of protein, nucleic acid, lipids, and carbohydrates.
  • It helps in
  1. a) Endocytosis: Degradation of macromolecule
  2. b) Autophagy: Degradation of unwanted intercellular constituents

 

Involved enzymes  Hydrolases, Acid phosphatase, Nucleases, Proteases, Lipases, Sulfatases, etc.

 

  1. Glyoxysomes ( Only fat-storing cells of plants)
  • Present in the cotyledon of fat-storing seed.
  • Helps in the conversion of stored fat into carbohydrate
  • Operate the series of reactions known as the glyoxylate cycle

 

Enzymes involved: Citrate synthetase, Aconitase, Isocitrate lyase, malate synthetase, etc.

 

  1. Chloroplast
  • Contains two-unit membranes, outer and inner lipoprotineous membranes with intermembrane space.
  • Intermembrane encloses stroma or matrix.
  • Thylaknoid vesicles help absorb light energy and convert it to ATP.
  • Stroma is the CO2 reduction site to form starch and glucose.
  • Contains DNA which encodes some of the chloroplasts.

 

Enzyme involved: DNA polymerase, ribulose-1,5-biphosphate, Carboxylase oxygenase.

 

  1. Vacuole
  • Helps in removing waste materials implants.
  • The surrounding membrane tonoplast regulates the entry of ions into the vacuole.
  • Cell sap contains digestive enzymes that degrade and recycle macromolecular components no longer in use.
  • Anthocyanin gives color to flowers and fruits.

 

Function

  • Maintain cell turgidity
  • Storage function
  • Act as lysosome

Peroxisomes

  1. s
  • Convert toxic hydrogen peroxide into harmless H2O and O2.
  • Present in photosynthetic cells of higher plants and non-photosynthetic cells in gymnosperm, and pteridophytes.
  • Helps in photorespiration.

 

Involved enzymes: Catalyse, Glycollate oxidase, glutamate, urate oxidase, etc.

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