Gel-Electrophoresis

• Gel electrophoresis is a method for the separation and analysis of macromolecules (DNA, RNA, and proteins) and their fragments, based on their size and charge.
• It is used in clinical chemistry to separate proteins by charge and/or size (IEF agarose, essentially size independent) and in biochemistry and molecular biology to separate a mixed population of DNA and RNA fragments by length, to estimate the size of DNA and RNA fragments or to separate proteins by charge.
• Gel electrophoresis uses a gel as an anticonvective medium and/or sieving medium during electrophoresis, the movement of a charged particle in an electrical field.